GC/MS analysis of hypoxic volatile metabolic markers in the MDA-MB-231 breast cancer cell line.

ABSTRACT: Hypoxia in disease describes persistent low oxygen conditions, observed in a range of pathologies, including cancer. In the discovery of biomarkers in biological models, pathophysiological traits present a source of translatable metabolic products for the diagnosis of disease in humans. Part of the metabolome is represented by its volatile, gaseous fraction; the volatilome. Human volatile profiles, such as those found in breath, are able to diagnose disease, however accurate volatile biomarker discovery is required to target reliable biomarkers to develop new diagnostic tools. Using custom chambers to control oxygen levels and facilitate headspace sampling, the MDA-MB-231 breast cancer cell line was exposed to hypoxia (1% oxygen) for 24 h. The maintenance of hypoxic conditions in the syste

SUBMITTER: Issitt T 

PROVIDER: S-EPMC10210155 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature