Lyophilization Reduces Aggregation of Three-Dimensional DNA Origami at High Concentrations.

ABSTRACT: Although for many purposes, low concentrations of DNA origami are sufficient, certain applications such as cryo electron microscopy, measurements involving small-angle X-ray scattering, or in vivo applications require high DNA origami concentrations of >200 nM. This is achievable by ultrafiltration or polyethylene glycol precipitation but often at the expense of increasing structural aggregation due to prolonged centrifugation and final redispersion in low buffer volumes. Here, we show that lyophilization and subsequent redispersion in low buffer volumes can achieve high concentrations of DNA origami while drastically reducing aggregation due to initially very low DNA origami concentrations in low salt buffers. We demonstrate this for four structurally different types of three-dimen

SUBMITTER: Baptist AV 

PROVIDER: S-EPMC10210204 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature