Magnetic-activated cell sorting identifies a unique lung microbiome community.

ABSTRACT:

Background

The advent of culture-independent, next-generation DNA sequencing has led to the discovery of distinct lung bacterial communities. Studies of lung microbiome taxonomy often reveal only subtle differences between health and disease, but host recognition and response may distinguish the members of similar bacterial communities in different populations. Magnetic-activated cell sorting has been applied to the gut microbiome to identify the numbers and types of bacteria eliciting a humoral response. We adapted this technique to examine the populations of immunoglobulin-bound bacteria in the lung.

Methods

Sixty-four individuals underwent bronchoalveolar lavage (BAL). We separated immunoglobulin G-bound bacteria using magnetic-activated cell sorting and sequenced the 16S

SUBMITTER: Dunlap DG 

PROVIDER: S-EPMC10210470 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature