Thermodynamic Compensation in Peptides Following Liquid-Liquid Phase Separation.

ABSTRACT: Liquid-liquid phase separation of proteins often incorporates intrinsically disordered proteins or those with disordered regions. Examining these processes via the entropy change is desirable for establishing a quantitative foundation with which to probe and understand these phase transitions. Of interest is the effect of residue sequence on the entropy of the peptide backbone. In this work we model these systems via all atom simulations of liquid-liquid phase separation of peptides. Systems of supersaturated pentapeptides separate into a peptide-dense liquid droplet phase as well as a dilute (saturated) aqueous phase. An analysis of the change in backbone conformational entropy associated with the phase transition was performed. We examined systems of four different pentapeptides (GGGGG,

SUBMITTER: Workman RJ 

PROVIDER: S-EPMC10210544 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature