Highly efficient cell-microbead encapsulation using dielectrophoresis-assisted dual-nanowell array.

ABSTRACT: Recent advancements in micro/nanofabrication techniques have led to the development of portable devices for high-throughput single-cell analysis through the isolation of individual target cells, which are then paired with functionalized microbeads. Compared with commercially available benchtop instruments, portable microfluidic devices can be more widely and cost-effectively adopted in single-cell transcriptome and proteome analysis. The sample utilization and cell pairing rate (∼33%) of current stochastic-based cell-bead pairing approaches are fundamentally limited by Poisson statistics. Despite versatile technologies having been proposed to reduce randomness during the cell-bead pairing process in order to statistically beat the Poisson limit, improvement of the overall pairing rate of a

SUBMITTER: Tian Z 

PROVIDER: S-EPMC10210622 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature