Molecular Characterization of Clarithromycin Resistance in Helicobacter pylori Strains.

ABSTRACT:

Background

The purpose of this study was to determine the antimicrobial status of stocked clinical Helicobacter pylori isolates by using antibiotic gradient test and subsequently identify the mutations that cause clarithromycin resistance by DNA sequencing. Turkey is a transition zone between Europe and Asia; therefore, we also aimed to show both continents' mutations in Turkish isolates.

Methods

One hundred forty-seven H. pylori isolates that had been stocked at -80°C between 1998 and 2008 were randomly selected and included in the study. Antibiotic susceptibility tests were performed using antibiotic gradient test for clarithromycin, amoxicillin, tetracycline, metronidazole, and levofloxacin. A polymerase chain reaction targeting the region of 23S rRNA gene domain V of H.

SUBMITTER: Orsten S 

PROVIDER: S-EPMC10210630 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature