Antiviral and protective effect of small interfering RNAs against rift valley fever virus in vitro.

ABSTRACT:

Background

Rift Valley Fever Virus (RVFV) is an arbovirus, a zoonotic disease that resurfaces as a potential hazard beyond geographic boundaries. Fever that can proceed to encephalitis, retinitis, hemorrhagic fever, and death is the main manifestation observed in human infections. RVFV has no authorized medication. The RNA interference (RNAi) gene silencing pathway is extremely well conserved. By targeting specific genes, small interfering RNA (siRNA) can be used to suppress viral replication. The aim of this study was to design specific siRNAs against RVFV and evaluate their prophylactic and antiviral effects on the Vero cells.

Methods and results

Various siRNAs were designed using different bioinformatics tools. Three unique candidates were tested against an Egyptian sheep

SUBMITTER: Ahmed EM 

PROVIDER: S-EPMC10211306 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature