Detection of Burkholderia pseudomallei with CRISPR-Cas12a based on specific sequence tags.

ABSTRACT: Melioidosis is a bacterial infection caused by Burkholderia pseudomallei (B. pseudomallei), posing a significant threat to public health. Rapid and accurate detection of B. pseudomallei is crucial for preventing and controlling melioidosis. However, identifying B. pseudomallei is challenging due to its high similarity to other species in the same genus. To address this issue, this study proposed a dual-target method that can specifically identify B. pseudomallei in less than 40 min. We analyzed 1722 B. pseudomallei genomes to construct large-scale pan-genomes and selected specific sequence tags in their core genomes that effectively distinguish B. pseudomallei from its closely related species. Specifically, we selected two specific tags, LC1

SUBMITTER: Zhang JX 

PROVIDER: S-EPMC10211466 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature