Rab8, Rab11, and Rab35 coordinate lumen and cilia formation during zebrafish left-right organizer development.

ABSTRACT: An essential process during Danio rerio's left-right organizer (Kupffer's Vesicle, KV) formation is the formation of a motile cilium by developing KV cells which extends into the KV lumen. Beating of motile cilia within the KV lumen directs fluid flow to establish the embryo's left-right axis. However, the timepoint at which KV cells start to form cilia and how cilia formation is coordinated with KV lumen formation have not been examined. We identified that nascent KV cells form cilia at their centrosomes at random intracellular positions that then move towards a forming apical membrane containing cystic fibrosis transmembrane conductance regulator (CFTR). Using optogenetic clustering approaches, we found that Rab35 positive membranes recruit Rab11 to modulate CFTR delivery to the apical m

SUBMITTER: Aljiboury AA 

PROVIDER: S-EPMC10212091 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature