NSUN2-mediated m⁵ C RNA methylation dictates retinoblastoma progression through promoting PFAS mRNA stability and expression.

ABSTRACT:

Background

The precise temporal and spatial regulation of N⁵ -methylcytosine (m⁵ C) RNA modification plays essential roles in RNA metabolism, and is necessary for the maintenance of epigenome homeostasis. Howbeit, the mechanism underlying the m⁵ C modification in carcinogenesis remains to be fully addressed.

Methods

Global and mRNA m⁵ C levels were determined by mRNA isolation and anti-m⁵ C dot blot in both retinoblastoma (RB) cells and clinical samples. Orthotopic intraocular xenografts were established to examine the oncogenic behaviours of RB. Genome-wide multiomics analyses were performed to identify the functional target of NSUN2, including proteomic analysis, transcriptome screening and m⁵ C-methylated R

SUBMITTER: Zuo S 

PROVIDER: S-EPMC10212275 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature