Peripheral blood mononuclear cell mitochondrial dysfunction in acute alcohol-associated hepatitis.

ABSTRACT:

Background

Patients with acute alcohol-associated hepatitis (AH) have immune dysfunction. Mitochondrial function is critical for immune cell responses and regulates senescence. Clinical translational studies using complementary bioinformatics-experimental validation of mitochondrial responses were performed in peripheral blood mononuclear cells (PBMC) from patients with AH, healthy controls (HC), and heavy drinkers without evidence of liver disease (HD).

Methods

Feature extraction for differentially expressed genes (DEG) in mitochondrial components and telomere regulatory pathways from single-cell RNAseq (scRNAseq) and integrated 'pseudobulk' transcriptomics from PBMC from AH and HC (n = 4 each) were performed. After optimising isolation and processing protocols for function

SUBMITTER: Bellar A 

PROVIDER: S-EPMC10212276 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature