In vitro construction of liver organoids with biomimetic lobule structure by a multicellular 3D bioprinting strategy.

ABSTRACT: Liver disease is one of the serious threats to human life and health. Three-dimensional (3D) liver models, which simulate the structure and function of natural liver tissue in vitro, have become a common demand in medical, scientific and pharmaceutical fields nowadays. However, the complex cellular composition and multi-scale spatial arrangement of liver tissue make it extremely challenging to construct liver models in vitro. According to HepaRG preference and printing strategy, the formulation of bioink system with opposite charge is optimized. The sodium alginate-based bioink 1 and dipeptide-based bioink 2 are used to ensure structural integrity and provide flexible designability, respectively. The HepaRG/HUVECs/LX-2-laden liver organoids with biomimetic lobule structure are fabricated b

SUBMITTER: Jian H 

PROVIDER: S-EPMC10212698 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature