Maximizing protein production by keeping cells at optimal secretory stress levels using real-time control approaches.

ABSTRACT: Optimizing the production of recombinant proteins is a problem of major industrial and pharmaceutical importance. Secretion of the protein by the host cell considerably simplifies downstream purification processes. However, for many proteins, this is also the limiting production step. Current solutions involve extensive engineering of the chassis cell to facilitate protein trafficking and limit protein degradation triggered by excessive secretion-associated stress. Here, we propose instead a regulation-based strategy in which induction is dynamically adjusted to an optimal strength based on the current stress level of the cells. Using a small collection of hard-to-secrete proteins, a bioreactor-based platform with automated cytometry measurements, and a systematic assay to quantify secrete

SUBMITTER: Sosa-Carrillo S 

PROVIDER: S-EPMC10212943 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature