A novel precision-serology assay for SARS-CoV-2 infection based on linear B-cell epitopes of Spike protein.

ABSTRACT:

Introduction

The COVID-19 pandemic illustrates the need for serology diagnostics with improved accuracy. While conventional serology based on recognition of entire proteins or subunits thereof has made significant contribution to the antibody assessment space, it often suffers from sub-optimal specificity. Epitope-based, high-precision, serology assays hold potential to capture the high specificity and diversity of the immune system, hence circumventing the cross-reactivity with closely related microbial antigens.

Methods

We herein report mapping of linear IgG and IgA antibody epitopes of the SARS-CoV-2 Spike (S) protein in samples from SARS-CoV-2 exposed individuals along with certified SARS-CoV-2 verification plasma samples using peptide arrays.

Results

We identifie

SUBMITTER: Lundin SB 

PROVIDER: S-EPMC10213285 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature