RpoZ regulates 2,4-DAPG production and quorum sensing system in Pseudomonas fluorescens 2P24.

ABSTRACT:

Introduction

Pseudomonas fluorescens 2P24 was isolated from soil of natural decay associated with wheat take-all and it can effectively control soil-borne diseases caused by a variety of plant pathogens. 2,4-diacetylphloroglucinol (2,4-DAPG), is produced by P. fluorescens 2P24 and plays an important role in the prevention and control of plant diseases. To understand the resistant mechanism, in this study, we conducted experiments to explore the regulation role of rpoZ in the synthesis of the antibiotic 2,4-DAPG and regulation of QS system.

Methods

A random mini-Tn5 mutagenesis procedure was used to screen regulators for phlA transcription in stain PM901, which containing a phlA∷lacZ transcriptional fusion reporter plasmid. We identified 12 inserti

SUBMITTER: Wei Y 

PROVIDER: S-EPMC10213339 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature