DMKPs provide a generalizable strategy for studying genes required for reproduction or viability in nontraditional model organisms.

ABSTRACT: The advent of CRISPR/Cas9-mediated genome editing has expanded the range of animals amenable to targeted genetic analysis. This has accelerated research in animals not traditionally studied using molecular genetics. However, studying genes essential for reproduction or survival in such animals remains challenging, as they lack the tools that aid genetic analysis in traditional genetic model organisms. We recently introduced the use of distinguishably marked knock-in pairs (DMKPs) as a strategy for rapid and reliable genotyping in such species. Here we show that DMKPs also facilitate the maintenance and study of mutations that cannot be maintained in a homozygous state, a group which includes recessive lethal and sterile mutations. Using DMKPs, we disrupt the zero population growth locus in

SUBMITTER: Laursen WJ 

PROVIDER: S-EPMC10213491 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature