An automated method to discover true events and classification of intracellular Ca2+ profiles for endothelium in situ injury assay

ABSTRACT: Introduction: Endothelial cells (ECs), being located at the interface between flowing blood and vessel wall, maintain cardiovascular homeostasis by virtue of their ability to integrate chemical and physical cues through a spatio-temporally coordinated increase in their intracellular Ca2+ concentration ([Ca2+]i). Endothelial heterogeneity suggests the existence of spatially distributed functional clusters of ECs that display different patterns of intracellular Ca2+ response to extracellular inputs. Characterizing the overall Ca2+ activity of the endothelial monolayer in situ requires the meticulous analysis of hundreds of ECs. This complex analysis consists in detecting and quantifying the true Ca2+ events associated to extracellular stimulation and classifying their intracellular Ca2+ prof

SUBMITTER: Sanchez-Tecuatl M 

PROVIDER: S-EPMC10213911 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature