Programming of Multicellular Patterning with Mechano-Chemically Microstructured Cell Niches.

ABSTRACT: Multicellular patterning of stem-cell-derived tissue models is commonly achieved via self-organizing activities triggered by exogenous morphogenetic stimuli. However, such tissue models are prone to stochastic behavior, limiting the reproducibility of cellular composition and forming non-physiological architectures. To enhance multicellular patterning in stem cell-derived tissues, a method for creating complex tissue microenvironments endowed with programmable multimodal mechano-chemical cues, including conjugated peptides, proteins, morphogens, and Young's moduli defined over a range of stiffnesses is developed. The ability of these cues to spatially guide tissue patterning processes, including mechanosensing and the biochemically driven differentiation of selected cell types, is demonstr

SUBMITTER: Newman PLH 

PROVIDER: S-EPMC10214222 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature