A genome-wide CRISPR screen maps endogenous regulators of PPARG gene expression in bladder cancer.

ABSTRACT: Peroxisome proliferator-activated receptor gamma (PPARγ) is a nuclear receptor central in the regulation of key cellular processes including cell metabolism, tissue differentiation, and regulation of the immune system. PPARγ is required for normal differentiation of the urothelium and is thought to be an essential driver of the luminal subtype of bladder cancer. However, the molecular components that regulate PPARG gene expression in bladder cancer remain unclear. Here, we developed an endogenous PPARG reporter system in luminal bladder cancer cells and performed genome-wide CRISPR knockout screening to identify bona fide regulators of PPARG gene expression. Functional validation of the dataset confirmed GATA3, SPT6, and the cohesin complex components SMC1A, and

SUBMITTER: Tortora D 

PROVIDER: S-EPMC10214290 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature