Over-expression of hsa_circ_0088214 suppresses tumor progression by inhibiting Akt signaling pathway in osteosarcoma.

ABSTRACT:

Background

To explore the effect of has_circ_0088214 in osteosarcoma cells and corresponding mechanisms.

Methods

Osteosarcoma cell line MG63 and U2OS were selected in this study. Wound-healing and matrigel transwell assays were performed to detect migration and invasion capacities. CCK-8 assay was used to measure cell growth and cisplatin resistance. Cell apoptosis was observed by Hoechst 33342 staining after H₂O₂ induce. Western Blot was used to detect protein expression level. The rescue experiments were also performed using an Akt activator SC79.

Results

Hsa_circ_0088214 was down-regulated in osteosarcoma cells compared to normal osteoblast cells. Over-expression of has_circ_0088214 significantly reduced osteosarcoma cells invasion, migration

SUBMITTER: Hao Z 

PROVIDER: S-EPMC10214608 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature