Phylogenetic analysis of HA and NA genes of influenza A viruses in immunosuppressed inpatients in Beijing during the 2018-2020 influenza seasons.

ABSTRACT:

Background

Influenza A viruses have undergone rapid evolution with virulent; however, complete and comprehensive data on gene evolution and amino acid variation of HA and NA in immunosuppressed patients was few. In this study, we analysed molecular epidemiology and evolution of influenza A viruses in immunosuppressed population, and immunocompetent population were used as controls.

Methods

Full sequences of HA and NA of A(H1N1)pdm09 and A(H3N2) were acquired through reverse transcription-polymerase chain reaction (RT-PCR). HA and NA genes were sequenced using the Sanger method and phylogenetically analysed using ClustalW 2.10 and MEGA software version 11.0.

Results

During the 2018-2020 influenza seasons, 54 immunosuppressed and 46 immunocompetent inpatients screened p

SUBMITTER: Liu Y 

PROVIDER: S-EPMC10215044 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature