Development of an Efficient FRET-Based Ratiometric Uranium Biosensor.

ABSTRACT: The dispersion of uranium in the environment can pose a problem for the health of humans and other living organisms. It is therefore important to monitor the bioavailable and hence toxic fraction of uranium in the environment, but no efficient measurement methods exist for this. Our study aims to fill this gap by developing a genetically encoded FRET-based ratiometric uranium biosensor. This biosensor was constructed by grafting two fluorescent proteins to both ends of calmodulin, a protein that binds four calcium ions. By modifying the metal-binding sites and the fluorescent proteins, several versions of the biosensor were generated and characterized in vitro. The best combination results in a biosensor that is affine and selective for uranium compared to metals such as calcium or

SUBMITTER: Sauge-Merle S 

PROVIDER: S-EPMC10216315 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature