Role of the MDR Efflux Pump AcrAB in Epithelial Cell Invasion by Shigella flexneri.

ABSTRACT: The tripartite complex AcrAB-TolC is the major RND pump in Escherichia coli and other Enterobacteriaceae, including Shigella, the etiological agent of bacillary dysentery. In addition to conferring resistance to many classes of antibiotics, AcrAB plays a role in the pathogenesis and virulence of several bacterial pathogens. Here, we report data demonstrating that AcrAB specifically contributes to Shigella flexneri invasion of epithelial cells. We found that deletion of both acrA and acrB genes causes reduced survival of S. flexneri M90T strain within Caco-2 epithelial cells and prevents cell-to-cell spread of the bacteria. Infections with single deletion mutant strains indicate that both AcrA and AcrB favor the viability of the intracellular bacter

SUBMITTER: Coluccia M 

PROVIDER: S-EPMC10216353 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature