A Convenient All-Cell Optical Imaging Method Compatible with Serial SEM for Brain Mapping.

ABSTRACT: The mammalian brain, with its complexity and intricacy, poses significant challenges for researchers aiming to understand its inner workings. Optical multilayer interference tomography (OMLIT) is a novel, promising imaging technique that enables the mapping and reconstruction of mesoscale all-cell brain atlases and is seamlessly compatible with tape-based serial scanning electron microscopy (SEM) for microscale mapping in the same tissue. However, currently, OMLIT suffers from imperfect coatings, leading to background noise and image contamination. In this study, we introduced a new imaging configuration using carbon spraying to eliminate the tape-coating step, resulting in reduced noise and enhanced imaging quality. We demonstrated the improved imaging quality and validated its applicabil

SUBMITTER: Wang T 

PROVIDER: S-EPMC10216590 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature