Oscillatory-Flow PCR Microfluidic Chip Driven by Low Speed Biaxial Centrifugation.

ABSTRACT: PCR is indispensable in basic science and biotechnology for in-orbit life science research. However, manpower and resources are limited in space. To address the constraints of in-orbit PCR, we proposed an oscillatory-flow PCR technique based on biaxial centrifugation. Oscillatory-flow PCR remarkably reduces the power requirements of the PCR process and has a relatively high ramp rate. A microfluidic chip that could perform dispensing, volume correction, and oscillatory-flow PCR of four samples simultaneously using biaxial centrifugation was designed. An automatic biaxial centrifugation device was designed and assembled to validate the biaxial centrifugation oscillatory-flow PCR. Simulation analysis and experimental tests indicated that the device could perform fully automated PCR amplifica

SUBMITTER: Fan Y 

PROVIDER: S-EPMC10216608 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature