Interactions of Amino Group Functionalized Tetraphenylvinyl and DNA: A Label-Free "On-Off-On" Fluorescent Aptamer Sensor toward Ampicillin.

ABSTRACT: As a type of aggregation-induced emission (AIE) fluorescent probe, tetraphenylvinyl (TPE) or its derivatives are widely used in chemical imaging, biosensing and medical diagnosis. However, most studies have focused on molecular modification and functionalization of AIE to enhance the fluorescence emission intensity. There are few studies on the interaction between aggregation-induced emission luminogens (AIEgens) and nucleic acids, which was investigated in this paper. Experimental results showed the formation of a complex of AIE/DNA, leading to the quenching of the fluorescence of AIE molecules. Fluorescent test experiments with different temperatures proved that the quenching type was static quenching. The quenching constants, binding constants and thermodynamic parameters demonstrated t

SUBMITTER: Geng W 

PROVIDER: S-EPMC10216731 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature