Cycle Threshold (Ct) Values of SARS-CoV-2 Detected with the GeneXpert^® System and a Mutation Associated with Different Target Gene Failure.

ABSTRACT: SARS-CoV-2 nucleic acid detection tests enable rapid virus detection; however, it is challenging to identify genotypes to comprehend the local epidemiology and infection routes in real-time qRT-PCR. At the end of June 2022, our hospital experienced an in-hospital cluster of COVID-19. When examined using the GeneXpert^® System, the cycle threshold (Ct) value of the N2 region of the nucleocapsid gene of SARS-CoV-2 was approximately 10 cycles higher than that of the envelope gene. Sanger sequencing revealed a G29179T mutation in the primer and probe binding sites. A review of past test results revealed differences in Ct values in 21 of 345 SARS-CoV-2-positive patients, of which 17 cases were cluster-related and 4 were not. Including these 21 cases, 36 cases in total were selected fo

SUBMITTER: Yamashita K 

PROVIDER: S-EPMC10217001 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature