A Method to Directly Identify Cronobacter sakazakii in Liquid Medium by MALDI-TOF MS.

ABSTRACT: Matrix-assisted laser desorption ionization time-of-flight mass spectrometry has been widely used as an emerging technology for the rapid identification of microorganisms. Cronobacter sakazakii (C. sakazakii) is a food-borne pathogen of particular importance to the powdered infant formula (PIF) processing environment due to its high lethality in infants. However, the traditional solid spotting detection method of pretreating samples for MALDI-TOF MS leads only to qualitative detection of C. sakazakii. We developed a new, low-cost, robust liquid spotting pretreatment method and used a response surface methodology to optimize its parameters. The applicability, accuracy, and quantitative potential were measured for different types of samples. The optimal parameters of this metho

SUBMITTER: Song D 

PROVIDER: S-EPMC10217744 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature