Functional Identification and Genetic Transformation of the Ammonium Transporter PtrAMT1;6 in Populus.

ABSTRACT: The ammonium transporter (AMT) family gene is an important transporter involved in ammonium uptake and transfer in plants and is mainly engaged in the uptake and transport of ammonium from the environment by roots and the reabsorption of ammonium in the aboveground parts. In this study, the expression pattern, functional identification, and genetic transformation of the PtrAMT1;6 gene, a member of the ammonium transporter protein family in P. trichocarpa, were investigated as follows: (1) Fluorescence quantitative PCR demonstrated that the PtrAMT1;6 gene was preferentially expressed in the leaves, with both dark-induced and light-inhibited expression patterns. (2) A functional restoration assay using the yeast ammonium transporter protein mutant strain indicated that t

SUBMITTER: Yang C 

PROVIDER: S-EPMC10218248 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature