Identification of Endometrial Cancer-Specific microRNA Biomarkers in Endometrial Fluid.

ABSTRACT: Abnormal uterine bleeding is a common benign gynecological complaint and is also the most common symptom of endometrial cancer (EC). Although many microRNAs have been reported in endometrial carcinoma, most of them were identified from tumor tissues obtained at surgery or from cell lines cultured in laboratories. The objective of this study was to develop a method to detect EC-specific microRNA biomarkers from liquid biopsy samples to improve the early diagnosis of EC in women. Endometrial fluid samples were collected during patient-scheduled in-office visits or in the operating room prior to surgery using the same technique performed for saline infusion sonohysterography (SIS). The total RNA was extracted from the endometrial fluid specimens, followed by quantification, reverse transcript

SUBMITTER: Yang J 

PROVIDER: S-EPMC10218319 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature