TNFα Causes a Shift in Gene Expression of TNFRSF1A and TNFRSF1B Isoforms.

ABSTRACT: Alternative splicing is a part of mRNA processing that expands the diversity of proteins encoded by a single gene. Studying the full range of proteins-products of translation of alternatively spliced mRNA is extremely important for understanding the interactions between receptor proteins and ligands since different receptor protein isoforms can provide variation in the activation of signaling pathways. In this study, we investigated the expression of isoforms of TNFR1 and TNFR2 receptors before and after exposure to TNFα in two cell lines that had previously demonstrated diverse effects on cell proliferation under TNFα incubation using RT-qPCR. We found that after incubation with TNFα: (1) expression of isoform 3 of the TNFRSF1A gene was increased in both cell lines; (2) the cell li

SUBMITTER: Perik-Zavodskaia O 

PROVIDER: S-EPMC10218394 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature