Evaluation of Library Preparation Workflows and Applications to Different Sample Types Using the PowerSeq^® 46GY System with Massively Parallel Sequencing.

ABSTRACT: This project evaluated the prototype PowerSeq^® 46GY System using donor DNA and casework-type samples. The goal of this study was to determine whether modifications to the manufacturer's protocol could increase read coverage and improve sample results. Buccal and casework-type libraries were prepared using the TruSeq^® DNA PCR-Free HT kit or the KAPA HyperPrep kit. Both kits were evaluated unmodified, and by substituting AMPure^® XP beads for the beads of the most optimal kit. Two qPCR kits, the PowerSeq^® Quant MS System and KAPA Library Quantification Kit, were also evaluated along with a KAPA size-adjustment workbook, which was compared as a third quantification method. Libraries were sequenced using the MiSeq^® FGx and data were analyze

SUBMITTER: Elwick K 

PROVIDER: S-EPMC10218485 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature