Freezing Does Not Alter Sperm Telomere Length despite Increasing DNA Oxidation and Fragmentation.

ABSTRACT: Correlations were reported between sperm telomere length (STL) and male fertility, sperm DNA fragmentation, and oxidation. Sperm freezing is widely used for assisted reproductive techniques, fertility preservation, and sperm donation. However, its impact on STL remains unknown. For this study, semen surplus from patients who underwent routine semen analysis were used. The impact of slow freezing on STL was analyzed by performing qPCR before and after freezing. Sperm populations with different STL were evaluated using Q-FISH. The relationship between sperm DNA oxidation, DNA fragmentation, and STL was assessed in fresh and frozen sperm samples. No significant impact of slow freezing on STL was observed, neither measured by qPCR nor Q-FISH. However, Q-FISH allowed for the distinguishing of s

SUBMITTER: Gouhier C 

PROVIDER: S-EPMC10218602 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature