Prevalence and molecular characterization of Entamoeba moshkovskii in diarrheal patients from Eastern India.

ABSTRACT:

Background

Importance of the amphizoic amoeba Entamoeba moshkovskii is increasing in the study of amoebiasis as a common human pathogen in some settings. Limited studies are found on the genetic and phylogenetic characterization of E. moshkovskii from India; hence remain largely unknown. In this study, we determined the prevalence and characterized the E. moshkovskii isolates in eastern India.

Methods

A three-year systemic surveillance study among a total of 6051 diarrhoeal patients from ID Hospital and BC Roy Hospital, Kolkata was conducted for E. moshkovskii detection via a nested PCR system targeting 18S rRNA locus. The outer primer set detected the genus Entamoeba and the inner primer pair identified the E. moshkovskii species. The 18S rRNA locus of the positive samples

SUBMITTER: Sardar SK 

PROVIDER: S-EPMC10218735 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature