Formation of a Purple Product upon the Reaction of ABTS Radicals with Proteins.

ABSTRACT: The reaction of the 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) free radical (ABTS^●) with proteins (bovine serum albumin, blood plasma, egg white, erythrocyte membranes, and Bacto Peptone) leads not only to a reduction of ABTS^● but also to the appearance of a purple color (absorption maximum at 550-560 nm). The aim of this study was to characterize the formation and explain the nature of the product responsible for the appearance of this color. The purple color co-precipitated with protein, and was diminished by reducing agents. A similar color was generated by tyrosine upon reaction with ABTS^●. The most feasible explanation for the color formation is the addiction of ABTS^● to proteins' tyrosine residues. The product formation was decrease

SUBMITTER: Kut K 

PROVIDER: S-EPMC10218885 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature