Exploration of the Strategy for Improving the Expression of Heterologous Sweet Protein Monellin in Aspergillus niger.

ABSTRACT: Aspergillus niger is a primary cell factory for food-grade protein (enzyme) production due to its strong protein secretion capacity and unique safety characteristics. The bottleneck issue for the current A. niger expression system is the difference in expression yield of heterologous proteins of non-fungal origin compared to those of fungal origin, which is about three orders of magnitude. The sweet protein monellin, derived from West African plants, has the potential to become a food-grade sweetener due to its high sweetness and the benefit of not containing sugar itself, but it is extremely difficult to establish a research model for heterologous expression in A. niger, owing to extremely low expression, a small molecular weight, and being undetectable with conventio

SUBMITTER: Li K 

PROVIDER: S-EPMC10218968 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature