Identification, Culture Characteristics and Whole-Genome Analysis of <i>Pestalotiopsis neglecta</i> Causing Black Spot Blight of <i>Pinus sylvestris</i> var. <i>mongolica</i>.

ABSTRACT: Black spot needle blight is a serious conifer disease of Pinus sylvestris var. mongolica occurring in Northeast China, which is usually caused by the plant pathogenic fungus Pestalotiopsis neglecta. From the diseased pine needles collected in Honghuaerji, the P. neglecta strain YJ-3 was isolated and identified as the phytopathogen, and its culture characteristics were studied. Then, we generated a highly contiguous 48.36-Mbp genome assembly (N50 = 6.62 Mbp) of the P. neglecta strain YJ-3 by combining the PacBio RS II Single Molecule Real Time (SMRT) and Illumina HiSeq X Ten sequencing platforms. The results showed that a total of 13,667 protein-coding genes were predicted and annotated using multiple bioinformatics databases. The genome assembly and annot

SUBMITTER: Yang J 

PROVIDER: S-EPMC10219049 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature