An Optimized Flow Cytometric Method to Demonstrate the Differentiation Stage-Dependent Ca²⁺ Flux Responses of Peripheral Human B Cells.

ABSTRACT: Calcium (Ca²⁺) flux acts as a central signaling pathway in B cells, and its alterations are associated with autoimmune dysregulation and B-cell malignancies. We standardized a flow-cytometry-based method using various stimuli to investigate the Ca²⁺ flux characteristics of circulating human B lymphocytes from healthy individuals. We found that different activating agents trigger distinct Ca²⁺ flux responses and that B-cell subsets show specific developmental-stage dependent Ca²⁺ flux response patterns. Naive B cells responded with a more substantial Ca²⁺ flux to B cell receptor (BCR) stimulation than memory B cells. Non-switched memory cells responded to anti-IgD stimulation with a naive-like Ca²⁺ flux pattern, whereas their

SUBMITTER: Bajnok A 

PROVIDER: S-EPMC10219102 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature