Hamster Sperm Possess Functional Na⁺/Ca²⁺-Exchanger 1: Its Implication in Hyperactivation.

ABSTRACT: Previous studies demonstrated that hamster sperm hyperactivation is suppressed by extracellular Na⁺ by lowering intracellular Ca²⁺ levels, and Na⁺/Ca²⁺-exchanger (NCX) specific inhibitors canceled the suppressive effects of extracellular Na⁺. These results suggest the involvement of NCX in the regulation of hyperactivation. However, direct evidence of the presence and functionality of NCX in hamster spermatozoa is still lacking. This study aimed to reveal that NCX is present and is functional in hamster spermatozoa. First, NCX1 and NCX2 transcripts were detected via RNA-seq analyses of hamster testis mRNAs, but only the NCX1 protein was detected. Next, NCX activity was determined by measuring the Na⁺-dependent Ca²⁺

SUBMITTER: Takei GL 

PROVIDER: S-EPMC10219178 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature