Characterization of a New Thermostable and Organic Solution-Tolerant Lipase from Pseudomonas fluorescens and Its Application in the Enrichment of Polyunsaturated Fatty Acids.

ABSTRACT: The search for and characterization of new lipases with excellent properties has always been urgent and is of great importance to meet industrial needs. In this study, a new lipase, lipB, from Pseudomonas fluorescens SBW25, belonging to the lipase subfamily I.3, was cloned and expressed in Bacillus subtilis WB800N. Enzymatic properties studies of recombinant LipB found that it exhibited the highest activity towards p-nitrophenyl caprylate at 40 °C and pH 8.0, retaining 73% of its original activity after incubation at 70 °C for 6 h. In addition, Ca²⁺, Mg²⁺, and Ba²⁺ strongly enhanced the activity of LipB, while Cu²⁺, Zn²⁺, Mn²⁺, and CTAB showed an inhibiting effect. The LipB also displayed noticeab

SUBMITTER: Hu Z 

PROVIDER: S-EPMC10219423 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature