Carrier-Mediated Process of Putrescine Elimination at the Rat Blood-Retinal Barrier.

ABSTRACT: Putrescine is a bioactive polyamine. Its retinal concentration is strictly controlled to maintain a healthy sense of vision. The present study investigated putrescine transport at the blood-retinal barrier (BRB) to gain a better understanding of the mechanisms of putrescine regulation in the retina. Our microdialysis study showed that the elimination rate constant during the terminal phase was significantly greater (1.90-fold) than that of [¹⁴C]D-mannitol, which is a bulk flow marker. The difference in the apparent elimination rate constants of [³H]putrescine and [¹⁴C]D-mannitol was significantly decreased by unlabeled putrescine and spermine, suggesting active putrescine transport from the retina to the blood across the BRB. Our study using model cell line

SUBMITTER: Tega Y 

PROVIDER: S-EPMC10219508 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature