Replication of Rocio virus in primary cultures of mouse neural cells.

ABSTRACT:

Objective

This study verified the replication efficiency of the Rocio virus in a primary culture of mouse neural cells.

Methods

Mixed primary cultures (neurons/glia) obtained from the brains of newborn isogenic BALB/c mice were inoculated with Rocio virus on the 7 th day of culture, and the development of cytopathogenic effects was monitored. The infection was confirmed via immunocytochemistry (anti-ROCV), while viral replication was quantified in infected primary cultures. The titration method used depended on the infection period.

Results

Rocio virus efficiently infected primary cultured neural cells, with the highest viral titer causing cytopathic changes was observed at 2 days post infection. The virus-infected primary culture survived for up to 7 days post infect

SUBMITTER: Souto ADP 

PROVIDER: S-EPMC10219622 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature