Deep ultraviolet fluorescence microscopy of three-dimensional structures in the mouse brain.

ABSTRACT: Three-dimensional (3D) imaging at cellular resolution improves our understanding of the brain architecture and is crucial for structural and functional integration as well as for the understanding of normal and pathological conditions in the brain. We developed a wide-field fluorescent microscope for 3D imaging of the brain structures using deep ultraviolet (DUV) light. This microscope allowed fluorescence imaging with optical sectioning due to the large absorption at the surface of the tissue and hence low tissue penetration of DUV light. Multiple channels of fluorophore signals were detected using single or a combination of dyes emitting fluorescence in the visible range of spectrum upon DUV excitation. Combination of this DUV microscope with microcontroller-based motorized stage enabled

SUBMITTER: Kasaragod DK 

PROVIDER: S-EPMC10219975 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature