Multimodal single cell analysis infers widespread enhancer co-activity in a lymphoblastoid cell line.

ABSTRACT: Non-coding regulatory elements such as enhancers are key in controlling the cell-type specificity and spatio-temporal expression of genes. To drive stable and precise gene transcription robust to genetic variation and environmental stress, genes are often targeted by multiple enhancers with redundant action. However, it is unknown whether enhancers targeting the same gene display simultaneous activity or whether some enhancer combinations are more often co-active than others. Here, we take advantage of recent developments in single cell technology that permit assessing chromatin status (scATAC-seq) and gene expression (scRNA-seq) in the same single cells to correlate gene expression to the activity of multiple enhancers. Measuring activity patterns across 24,844 human lymphoblastoid single

SUBMITTER: Ziyani C 

PROVIDER: S-EPMC10219981 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature