Computational structural-based GPCR optimization for user-defined ligand: Implications for the development of biosensors.

ABSTRACT: Organisms have developed effective mechanisms to sense the external environment. Human-designed biosensors exploit this natural optimization, where different biological machinery have been adapted to detect the presence of user-defined molecules. Specifically, the pheromone pathway in the model organism Saccharomyces cerevisiae represents a suitable candidate as a synthetic signaling system. Indeed, it expresses just one G-Protein Coupled Receptor (GPCR), Ste2, able to recognize pheromone and initiate the expression of pheromone-dependent genes. To date, the standard procedure to engineer this system relies on the substitution of the yeast GPCR with another one and on the modification of the yeast G-protein to bind the inserted receptor. Here, we propose an innovative computational procedu

SUBMITTER: Di Rienzo L 

PROVIDER: S-EPMC10220229 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature