Antibody toolkit to investigate eEF1A methylation dynamics in mRNA translation elongation.

ABSTRACT: Protein synthesis is a fundamental step in gene expression, with modulation of mRNA translation at the elongation step emerging as an important regulatory node in shaping cellular proteomes. In this context, five distinct lysine methylation events on eukaryotic elongation factor 1A (eEF1A), a fundamental nonribosomal elongation factor, are proposed to influence mRNA translation elongation dynamics. However, a lack of affinity tools has hindered progress in fully understanding how eEF1A lysine methylation impacts protein synthesis. Here we develop and characterize a suite of selective antibodies to investigate eEF1A methylation and provide evidence that methylation levels decline in aged tissue. Determination of the methyl state and stoichiometry on eEF1A in various cell lines by mass spect

SUBMITTER: Mealey-Farr R 

PROVIDER: S-EPMC10220242 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature