Apical-Out Human Airway Organoids Modeling SARS-CoV-2 Infection.

ABSTRACT: The respiratory epithelium, particularly the airway epithelium, is the primary infection site for respiratory pathogens. The apical surface of epithelial cells is constantly exposed to external stimuli including invading pathogens. Efforts have been made to establish organoid cultures to recapitulate the human respiratory tract. However, a robust and simple model with an easily accessible apical surface would benefit respiratory research. Here, we report the generation and characterization of apical-out airway organoids from the long-term expandable lung organoids that we previously established. The apical-out airway organoids morphologically and functionally recapitulated the human airway epithelium at a comparable level to the apical-in airway organoids. Moreover, apical-out airway organ

SUBMITTER: Chiu MC 

PROVIDER: S-EPMC10220522 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature