Comparative Evaluation of Commercial Protein A Membranes for the Rapid Purification of Antibodies.

ABSTRACT: Protein A chromatography is ubiquitous to antibody purification. The high specificity of Protein A for binding the Fc-region of antibodies and related products enables unmatched clearance of process impurities like host cell proteins, DNA, and virus particles. A recent development is the commercialization of research-scale Protein A membrane chromatography products that can perform capture step purification with short residence times (RT) on the order of seconds. This study investigates process-relevant performance and physical properties of four Protein A membranes: Purilogics Purexa™ PrA, Gore^® Protein Capture Device, Cytiva HiTrap™ Fibro PrismA, and Sartorius Sartobind^® Protein A. Performance metrics include dynamic binding capacity, equilibrium binding capacity, r

SUBMITTER: Osuofa J 

PROVIDER: S-EPMC10220532 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature