Biological Activity of Optimized Codon Bovine Type III Interferon Expressed in Pichia pastoris.

ABSTRACT: Type III interferons (IFN-λs) exhibit potent antiviral activity and immunomodulatory effects in specific cells. Nucleotide fragments of the bovine ifn-λ (boifn-λ) gene were synthetized after codon optimization. The boifn-λ gene was then amplified by splicing using overlap extension PCR (SOE PCR), resulting in the serendipitous acquisition of the mutated boIFN-λ3^V18M. The recombinant plasmid pPICZαA-boIFN-λ3/λ3^V18M was constructed, and the corresponding proteins were expressed in Pichia pastoris with a high-level extracellular soluble form. Dominant expression strains of boIFN-λ3/λ3^V18M were selected by Western blot and ELISA and cultured on a large scale, and the recombinant proteins purified by ammonium sulfate precipitation and

SUBMITTER: An R 

PROVIDER: S-EPMC10221290 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature