Gel-Free Tools for Quick and Simple Screening of Anti-Topoisomerase 1 Compounds.

ABSTRACT: With the increasing need for effective compounds against cancer or pathogen-borne diseases, the development of new tools to investigate the enzymatic activity of biomarkers is necessary. Among these biomarkers are DNA topoisomerases, which are key enzymes that modify DNA and regulate DNA topology during cellular processes. Over the years, libraries of natural and synthetic small-molecule compounds have been extensively investigated as potential anti-cancer, anti-bacterial, or anti-parasitic drugs targeting topoisomerases. However, the current tools for measuring the potential inhibition of topoisomerase activity are time consuming and not easily adaptable outside specialized laboratories. Here, we present rolling circle amplification-based methods that provide fast and easy readouts for sc

SUBMITTER: Keller JG 

PROVIDER: S-EPMC10221459 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature